Semaglutide Titration Math

Semaglutide ships as a 5 mg lyophilized vial — and 5 mg is a number researchers often trip over, because the reference titration stages are in micrograms. This guide walks the math from vial to schedule.

Concentration first

5 mg = 5,000 µg. Reconstitute with your chosen water volume and the concentration falls out:

• 2 mL water → 2,500 µg/mL
• 1 mL water → 5,000 µg/mL (doubles every unit value)

Smaller volumes mean denser solutions and tinier draws; larger volumes are easier to pipette. The semaglutide calculator handles any volume.

Unit conversion

On a U-100 syringe, 1 unit = 0.01 mL. At 2,500 µg/mL:

• 1 unit = 25 µg
• A 250 µg dose = 10 units
• A 500 µg dose = 20 units

The reference titration schedule on the calculator page lists week-by-week stages with their unit equivalents — it updates live if you change the water volume.

Vial yield

A 5,000 µg vial at a 250 µg dose yields 20 doses. That’s the number that drives reorder timing, and it’s why we recommend checking doses-per-vial before committing to a schedule.

Practical notes

Semaglutide is stored lyophilized at −20 °C, reconstituted with USP bacteriostatic water, and used per the Certificate of Analysis window. See Semaglutide for the full specification sheet.

For laboratory research use only. Not medical advice.