How much water should I add to a 5 mg semaglutide vial?

A common research convention is 1–2 mL of USP bacteriostatic water per 5 mg vial. The calculator accepts any volume and returns the resulting concentration and units per dose.

Why does the calculator show units, not mL?

Most research protocols are drawn with U-100 insulin syringes, where 1 unit = 0.01 mL. Units are the finer, more convenient scale for µg-level doses.

Is the titration schedule a dosing recommendation?

No. It is a reference arithmetic table for planning research protocols and is included for transparency. Vyroval does not provide dosing guidance of any kind.

5mg vial reconstitution & dosing

Laboratory Tools

Semaglutide Calculator in the vial.

Reconstitution and dose math for semaglutide 5mg research vials — water volume, concentration, and syringe units.

5 mg vial, exact units.

Semaglutide is supplied lyophilized in 5 mg vials. Add bacteriostatic water and read off the exact U-100 syringe units for any target dose, with a reference titration schedule for research planning.

Concentration

— µg/mL

Per 1 unit

— µg

Draw per dose

— units(—)

Doses per vial

—

Vials / week

—

Reference titration schedule

Phase	Dose	Units at current water
Week 1 (start)	250 µg	—
Week 5	500 µg	—
Week 9	1,000 µg	—
Week 13	1,700 µg	—

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How it works

The math behind the tool

Concentration

5,000 µg ÷ water (mL) = µg/mL

A 5 mg semaglutide vial contains 5,000 µg. With 2 mL of bacteriostatic water that is2,500 µg/mL — each unit holds 25 µg.

Reference kinetics

reported half-life ≈ 7 days (long-acting GLP-1)

Semaglutide is a long-acting GLP-1 receptor agonist and a common reference standard in receptor binding assays. Its reference half-life is used here only as arithmetic context.

Arithmetic only — for laboratory research use, not medical advice. Verify concentrations against the Certificate of Analysis shipped with each batch.

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